Three-photon microscopy has been increasingly adopted as a powerful tool for monitoring neural activities beyond the typical two-photon imaging depth. In this webinar hosted by the OSA Imaging Optical Design Technical Group, Tianyu Wang from Cornell University will outline the unique properties that differentiates three-photon from two-photon imaging in deep or turbid samples. In order to optimize three-photon microscopy for volumetric neuronal imaging, Wang will summarize the considerations on imaging parameter selection, including their effects on calcium imaging quality and their perturbation to the sample. Finally, Wang will cover the existing techniques for volumetric three-photon imaging and discuss their prospects for mesoscale imaging.
What You Will Learn:
• The basics of multi-photon imaging
• The state-of-the art multi-photon functional imaging technologies
• The representative applications of multi-photon functional imaging in neuronscience
• The challenges and the future
Who Should Attend:
• Graduate students ,researchers, and engineers working in multi-photon functional imaging